Interplay of HIV-1 phenotype and neutralizing antibody response in pathogenesis of AIDS
✍ Scribed by Gabriella Scarlatti; Thomas Leitner; Vida Hodara; Marianne Jansson; Anders Karlsson; Johan Wahlberg; Paolo Rossi; Mathias Uhlén; Eva Maria Fenyö; Jan Albert
- Book ID
- 107908632
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 662 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0165-2478
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A mouse monoclonal hybridoma cell line producing IgG 1 k to human immunodeficiency virus (HIV-1) gp120 envelope protein was cultured in several systems. A small-scale flask culture was essential for characterizing the culture variables of the hybridoma. A dialysis tubing culture appeared to be an ex
## Abstract Antibodies to __E. coli__‐produced HIV‐1 __nef__, __rev__, __tat__, __vpu__, and __vpr__ proteins were measured by enzyme immunoassay in serial sets of sera from 72 men seroconverting for antibodies to HIV‐1 structural proteins, and from 190 initially symptom‐free men who were seroposit
Our objective was to analyse the humoral response to the major neutralizing epitopes of gp120. The kinetics of the appearance of antibodies directed to the V3 region (V3 Abs) and antibodies directed to the CD4 binding site (CD4BS Abs) were compared in sequential sera from 20 seroconverters. V3 Abs w