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Internal translation initiation in the design of improved expression vectors

✍ Scribed by Monique V. Davies; Randal J. Kaufman

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
691 KB
Volume
3
Category
Article
ISSN
0958-1669

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✦ Synopsis

The discovery of a novel, cap-independent mechanism of translation used by picornavirus mRNAs has led to new advances in the engineering of mammalian expression vectors. It is now possible to express several proteins in a coordinate fashion from a single mRNA. Improved expression vectors suitable for virus-mediated transfer and direct DNA transfer are described.

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