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Internal pH indicators for biomolecular NMR

✍ Scribed by Olga K. Baryshnikova; Thomas C. Williams; Brian D. Sykes

Book ID
106401543
Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
198 KB
Volume
41
Category
Article
ISSN
0925-2738

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## Abstract The intracellular pH (pHi) of a series of cancer cell lines was determined using the pH‐sensitive indicators imidazole (Im) or histidine (His) and diffusion‐weighted (DW) proton NMR spectroscopy. The DW method allows the observation at high magnetic field gradient values of only the slo