Interleukin 8 production of human leukocytes stimulated by triple or single helical conformer of an antitumor (1→3)-β-D-glucan preparation, sonifilan
✍ Scribed by Aiko Tsuzuki; Naohito Ohno; Yoshiyuki Adachi; Toshiro Yadomae
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 483 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0272-4391
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✦ Synopsis
Previous research has suggested that the immunopharmacological activity of soluble (1→3)β-D-glucan in mice depends on its conformation. In the present study, we examined the relationship between conformation of Sonifilan (SPG), a high molecular weight (1→3)-β-D-glucan, and interleukin 8 (IL-8) productivity in an in vitro human system. All experiments were performed using a polypropylene tube in order to reduce cellular adherence and reduce integrin-mediated signaling. SPG takes the form of a triple helical conformer in water, which changes in response to treatment with an aqueous sodium hydroxide to a single helical conformer (SPG-OH). Whole blood, peripheral blood mononuclear cells (PBMC), and neutrophils (PMN) were stimulated with either SPG or SPG-OH and then cultured in vitro. Next, IL-8 production of the cells and supernatants was measured immunochemically (ELISA and FACS). In whole blood and PBMC cultures, SPG-OH induced higher IL-8 production than SPG. In PMN culture, IL-8 production induced by SPG was comparable to that of SPG-OH. Enhanced IL-8 production of monocytes and PMN was confirmed by detection of increased levels of intracellular IL-8 cultured in the presence of blefeldin and then assessed by FACS. These findings suggested that SPG and SPG-OH enhanced the productivity of IL-8 in human leukocytes.