Interleukin-6 induces prostate cancer cell growth accompanied by activation of Stat3 signaling pathway
✍ Scribed by Lou, Wei; Ni, Zuyao; Dyer, Kevin; Tweardy, David J.; Gao, Allen C.
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 226 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0270-4137
No coin nor oath required. For personal study only.
✦ Synopsis
Background:
Interleukin-6 (il-6) is a pleiotropic cytokine that regulates growth and differentiation of various types of malignant tumors, including prostate carcinomas. the levels of il-6 are elevated in sera of patients with metastatic prostate cancer. in this study, we evaluate the role of il-6 in the growth regulation of prostate cancer cells.
Methods:
Expression of il-6 and its receptors in human prostate cancer cells was measured by elisa and rt-pcr. the effects of il-6 on cell growth were evaluated by ectopically expressing il-6 cdna into il-6-negative lncap human prostate cancer cells. stat3 dna binding activities were analyzed by electromobility shift assay and supershift assay.
Results:
Expression of il-6 was detected in the androgen-insensitive prostate cancer cell lines (i.e. , tsu, pc3, and du145), but not in the androgen-sensitive lncap cell line. il-6 receptors, including both il-6-specific receptor alpha chain and gp130 signal transducer, are expressed in all human prostate cancer cell lines (i.e., lncap, tsu, pc3, and du145). overexpression of il-6 by ectopically expressing il-6 into il-6-negative lncap human prostate cancer cells significantly increased clonogenic ability and cell proliferation in vitro compared to the il-6-negative parental lncap cells and the antisense controls. this growth stimulation by il-6 was accompanied by activation of the stat3 signaling transduction pathway.
Conclusions:
Il-6 is an autocrine growth factor for lncap human prostate cancer cells; the effects of il-6 on prostate cancer cell growth are mediated through the janus kinase-signal transducers and activators of transcription (jak-stat) signaling pathway.