Interleukin 4 inhibits murine osteoclast formation in vitro

✍ Scribed by Atsushi Shioi; Steven L. Teitelbaum; F. Patrick Ross; Howard G. Welgus; Hidenori Suzuki; Junichi Ohara; David L. Lacey

• Book ID: 102878622
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 593 KB
• Volume: 47
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240470313

✦ Synopsis

Interleukin 4 (IL-4) is a product of activated T cells and mast cells with effects on immunologic and hematopoietic processes. We now report that IL-4 inhibits the formation of osteoclasts from murine bone marrow <:ells cocultured with stromal cells. Numerous (3,000-4,000 cells/2 cm2) tartrate-resistant acid-phosphatase-positive multinucleated cells with the capacity to generate CAMP in response to salmon calcitonin (ED, , = lo-'' M) developed within 10-12 days of culture. IL-4 (ID5,, = 10 U/ml) inhibited osteoclast generation in doses similar to those that induce proliferation of IL-4-responsive T cells. Additionally, the rat antimurine IL-4 monoclonal antibody 11 B11 antagonizes the IL-4-inhibitory effect on osteoclast formation. These results suggest that IL-4 impedes agonist-induced in vitro bone resorption by inhibiting osteoclastogenesis.