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Interleukin-4 and -5 as modulators of nerve growth factor synthesis/secretion in astrocytes

✍ Scribed by H. Awatsuji; Y. Furukawa; M. Hirota; Y. Murakami; S. Nii; S. Furukawa; K. Hayashi

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
637 KB
Volume
34
Category
Article
ISSN
0360-4012

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✦ Synopsis

To examine the regulation of nerve growth factor (NGF) gene expression with respect to neural trauma, we examined the effects of T cell-derived lymphokines on NGF synthesishecretion in cultured mouse astrocytes. Interleukin (1L)-4 and IL-5 significantly increased the amount of NGF secreted by astrocytes, whereas IL-2, IL-3, and IL-6 had no significant effect. IL-4 and IL-5 produced marked increases in NGF mRNA levels in astrocytes as demonstrated by the reverse transcription-polymerase chain reaction (RT-PCR) method. The effect of IL-4 and IL-5 was greater in quiescent astrocytes than in growing cells. Neither increase in thymidine incorporation nor any morphological change was observed during the treatment with IL-4 and IL-5. The stimulatory effect of IL-4 and IL-5 on NGF synthesis was completely inhibited by the addition of anti-IL-4 monoclonal antibody and anti-IL-5 monoclonal antibody, respectively. The results indicate that IL-4 and IL-5 specifically trigger a cascade of events to regulate NGF synthesis in astrocytes, independent of cell growth.

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