Interleukin 3-stimulated proliferation is sensitive to pertussis toxin: Evidence for a guanyl nucleotide regulatory protein-mediated signal transduction mechanism
✍ Scribed by David J. Kelvin; M. Shreeve; C. McAuley; D. L. McLeod; G. Simard; J. A. Connolly
- Book ID
- 102880853
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 850 KB
- Volume
- 138
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
lnterleukin 3 (IL-3) stirnulates several biochemical and biological responses in IL-3-dependent tissue culture cells. We examined the possibility that guanyl nucleotide regulatory (G) proteins rriay transduce signals from IL-3 receptors. We report here that pertussis toxin (PT), which can covalently modify a subclass of G proteins, is capable of inhibiting IL-3-stimulated proliferation in a dose-dependent fashion. PT inhibiton of IL-+stiniulated proliferation could be overcome by using the C a + + ionophore A23187 in conjunction with TPA. PT cwuld also inhibit IL-%stimulated hexosc transport. In the absence of IL-3, hexose transport could be stimulated by introducing CTPyS into intact cells. From these data we propose that IL-3 receptors transduce signals via a PT-sensitive G protein(s).