Interleukin-2 receptorα gene expression in intestinal allograft rejection

✍ Scribed by Liwei Zhu; Pengzhi Wang; Yudong Luo; Ning Lu; Hao Wang

• Book ID: 101267817
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 28 KB
• Volume: 19
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/(sici)1098-2752(1999)19:7<311::aid-micr3>3.0.co;2-1

✦ Synopsis

This study was undertaken to determine whether the interleukin-2 receptor ␣ (IL-2R␣) coding gene could serve as a genetic marker of intestinal allograft rejection. The small bowel graft from Jinbai mice was heterotopically transplanted to BALB/c mice. Naive BALB/c mice and BALB/c mice with intestinal isografts were used as controls. The mRNA transcription level of the spleen cells harvested from the recipients and controls was measured with quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) on postoperative day (POD) 3 and routine pathology was stud-ied in these grafts. The IL-2R␣ mRNA transcription level in untreated allografts was significantly higher than that of controls on POD 3, when intestinal allograft rejection was not detected. These data suggest that the IL-2R␣ mRNA transcription level may reflect the early cellular immune response and this genetic marker may be of value in monitoring intestinal allograft rejection.