Interleukin-2 as a neurotrophic factor for supporting the survival of neurons cultured from various regions of fetal rat brain
✍ Scribed by H. Awatsuji; Y. Furukawa; M. Nakajima; S. Furukawa; K. Hayashi
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 600 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Interleukin(1L)-2 supported the survival and enhanced neurite extension of cultured hippocampal neurons prepared from embryonic 18-day-old rats. This neurotrophic effect was observed at concentrations of 2 to 200 U/ml, and almost all the neurons could survive for more than 2 days in the presence of 200 U/ml of IL-2. This viability-promoting effect of IL-2 on the neurons was completely blocked with anti-IL-2 antibodies. IL-2 also supported the survival of cultured cortical, striatal, and septal neurons. These results indicate that IL-2 has a survival-promoting effect on a wide variety of neurons. On the other hand, IL-2 did not affect the choline acetyltransferase (ChAT) activity of striatal neurons, suggesting that this cytokine does not act as a differentiation factor for striatal cholinergic neurons.