Interleukin-1β-induced expression of monocyte chemotactic protein-1 in the rabbit retina: An in situ and immunohistochemical study

✍ Scribed by Carolyn A. Cuff; Joan W. Berman; Celia F. Brosnan

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 763 KB
• Volume: 30
• Category: Article
• ISSN: 0894-1491
• DOI: 10.1002/(sici)1098-1136(200005)30:3<279::aid-glia7>3.0.co;2-6

✦ Synopsis

In this study, the temporal and spatial expression of the chemotactic factor monocyte chemotactic protein-1 (MCP-1) was examined in the rabbit retina after challenge with the proinflammatory cytokine interleukin-1␤ (IL-1). In these tissues, IL-1 induces an acute inflammatory response of the epiretinal vessels that peaks Ϸ24 h postintraocular injection (pi) with the cytokine. At 2 h after challenge with IL-1, MCP-1 mRNA was expressed by perivascular microglial cells and astrocytes that form the glial limitans. Protein analysis at 3 h pi with IL-1 confirmed these sites of MCP-1 expression. The intensity of the mRNA and protein signals increased at 6 h and at 24 h. At these time points, MCP-1 message and protein also were detected in infiltrating macrophages and, at the latest time point, in endothelial cells as well. These data support the conclusion that IL-1 provides a strong stimulus for the rapid expression of MCP-1 mRNA and protein in retinal tissues, and they further support the role of endogenous glial cells as important sources of mediators involved in the regulation of inflammation occurring within the nervous system.