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Interleukin-1β converting enzyme in murine Langerhans cells and epidermal-derived dendritic cell lines

✍ Scribed by Kiyoshi Ariizumi; Toshiyuki Kitajima; Paul R. Bergstresser; Akira Takashima

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
582 KB
Volume
25
Category
Article
ISSN
0014-2980

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✦ Synopsis

Interleukin (IL)

-lp plays an essential role in the induction of T cell-mediated immune responses in skin. Langerhans cells (LC), which constitutively express IL-lp mRNA, have been assumed to be the primary source of IL-lP in murine epidermis. The purpose of this study was to determine whether LC express mRNA for the IL-113 converting enzyme (ICE), a protease that is required for processing pro-IL-10 into an active form. Here, we report that both IL-lg and ICE mRNA are expressed by the Ia' population (i.e. LC) in murine epidermis. Moreover, murine epidermal-derived DC lines (XS series) also express both IL-1P and ICE mRNA, and they secrete relatively large amounts of IL-lp following lipopolysaccharide (LPS) stimulation. Finally. LPS-triggered IL-113 secretion by XS cells is blocked almost completely by the ICE inhibitor acetyl-Tyr-Val-Ala-Asp-CH20C(0)-[2,6-(CF,),]Ph. These results demonstrate that LC are the primary source of IL-113 within the epidermis, and suggest that the proinflammatory role of IL-113 may be regulated pharmacologically by ICE inhibitors in vivo.

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