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Interleukin-1α induces cyclooxygenase-2 expression in bone-derived endothelial cells

✍ Scribed by Takumi Nakagawa; Naoya Fujita; Tomoko Oh-Hara; Takahide Kurokawa; Kozo Nakamura; Takashi Tsuruo

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
144 KB
Volume
179
Category
Article
ISSN
0021-9541

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✦ Synopsis

Histological studies have suggested that vascular endothelial cells in bone are members of a complex network that regulates bone development and remodeling by producing soluble factors or by mediating cell-cell adhesion. To clarify the role of bone-derived endothelial cell lines (BDECs) in bone remodeling, we established several clones of BDECs from the femurs of BALB/c mice after transformation with the SV40 virus. Then we examined the response of these clones to interleukin-1␣ (IL-1␣). IL-1␣ is known to induce bone resorption in part by increasing the expression of cyclooxygenase-2 (COX-2) that is associated with the production of PGE 2 in osteoblast-lineage cells. Treating the primary and established BDECs with IL-1␣ induced COX-2 mRNA expression. A transcriptional activation assay revealed that the treatment with IL-1␣ increased COX-2 promoter activity in a dose-dependent manner, and IL-1␣ promoted COX-2 protein expression in BDECs. Treatment with IL-1␣ promoted PGE 2 production from BDECs in a dose-dependent manner. These results indicate that IL-1␣ stimulates PGE 2 synthesis largely by inducing BDECs to express COX-2. Because PGE 2 stimulates bone resorption, these vascular endothelial cells, as well as osteoblast cells, play important roles in bone remodeling.

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