Interleukin-12 delivered by biodegradable microspheres promotes the antitumor activity of human peripheral blood lymphocytes in a human head and neck tumor xenograft/SCID mouse model
✍ Scribed by M. Abraham Kuriakose; F-A Chen; Nejat K. Egilmez; Y.S. Jong; Edith Mathiowitz; Mark D. DeLacure; Wesley L. Hicks Jr; Thom L. Loree; Richard B. Bankert
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 138 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1043-3074
No coin nor oath required. For personal study only.
✦ Synopsis
Background. The role of cytokines in tumor regression is now well established. The major limitation for the clinical use of cytokines is the lack of a simple and effective protocol for the local and sustained delivery of cytokines to the tumor milieu. This study reports suppression of human head and neck squamous cell carcinoma (HNSCC) by human peripheral blood lymphocytes (HuPBL) following local, sustained delivery of interleukin-12 (IL-12) to tumors with biodegradable microspheres in a human/SCID mouse chimeric model.
Materials and Methods. Nondisrupted biopsy pieces (120 mg) of primary HNSCC were implanted s.c. into severe combined immunodeficient (SCID) mice and were expanded by serial passage in mice. Tumors were then titrated with different doses of allogeneic HuPBL by coengraftment of tumor pieces and HuPBL