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Interleukin-10 inhibits IgE-mediated nitric oxide synthase induction and cytokine synthesis in normal human keratinocytes

✍ Scribed by Pierre-André Bécherel; Liliane Le Goff; Sandra Ktorza; Fateh Ouaaz; Jean-Michel Mencia-Huerta; Bernard Dugas; Patrice Debré; M. Djavad Mossalayi; Michel Arock

Book ID: 102163217
Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 478 KB
Volume: 25
Category: Article
ISSN: 0014-2980
DOI: 10.1002/eji.1830251042

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✦ Synopsis

Interleukin-10 inhibits IgE-mediated nitric oxide synthase induction and cytokine synthesis in normal human keratinocytes

Human keratinocytes (HK) generate nitric oxide (NO) and proinflammatory mediators following activation with either IgE/anti-IgE immune complexes or a combination of lipopolysaccharide (LPS) and interferon-y (IFN-y). Recently, interleukin-10 (IL-10) has been shown to down-regulate various inflammatory responses and to be secreted by lymphocytes and dendritic cells during skin inflammatory reactions. We show here that IL-10 down-regulates the production of tumor necrosis factor (TNF)-a and IL-6 by activated HK. Also, induction of inducible nitric oxide synthase (iNOS) expression in HK by IgE/anti-IgE or LPS/ IFN-y is significantly reduced by the addition of IL-10. This effect is dose dependent and correlates with reduction of iNOS mRNA production and enzyme level. Therefore, IL-10 down-regulates NO-mediated HK inflammatory responses and may thus participate in the regulation of the skin immune network.

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