Interleukin-10 inhibits cytokine generation from mast cells

✍ Scribed by Michel Arock; Claudia Zuany-Amorim; Monique Singer; Marc Benhamou; Marina Pretolani

• Book ID: 102163238
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 464 KB
• Volume: 26
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830260126

✦ Synopsis

Interleukin-10 inhibits cytokine generation from mast cells

This report examines the effect of recombinant murine interleukin-10 (rmIL-10) on antigen-induced P-hexosaminidase, leukotriene (LT)C, and cytokine release from mouse bone marrow-derived mast cells (BMMC). BMMC sensitized to hapten-monoclonal IgE directed against dinitrophenol-bovine serum albumin (DNP-BSA) and challenged with 10 ng/ml DNP-BSA generated phexosaminidase and LTC,-like material, which was followed by tumor necrosis factor-a (TNF-u) and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA expression and protein release. Incubation of BMMC with 1-100 ng/ml rmIL-10 inhibited cytokine generation, without affecting phexosaminidase and LTC4-like material release. TNF-u, but not GM-CSF mRNA expression, was also diminished in rmIL-10-treated BMMC, suggesting that down-regulation of cytokine production by rmIL-10 involves different mechanisms. These results identify a novel biological action of IL-10 as an inhibitor of cytokine production by stimulated mast cells.