Interleukin 1 stimulates prostaglandin synthesis and cyclic AMP accumulation in Swiss 3T3 fibroblasts: Interactions between two second messenger systems
✍ Scribed by Ronald M. Burch; Marsha F. White; Jane R. Connor
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 512 KB
- Volume
- 139
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Biochemical events elicited by interleukin 1 (IL-1) were studied in Swiss 3T3 fibroblasts. One hour after its addition, ILL1 stimulated synthesis of prostaglandin EL (PGE2), which continued to accumulate for 4 days. IL-1 also stimulated cAMP accumulation. lndomethacin blocked cAMP accumulation in response to IL-I , suggesting that PGE2 was responsible for the increase. Addition of exogneous PGEz to indomethacin-treated cells restored cAMP accumulation. IL-1 enhanced thymidine incorporation, and indomethacin attenuated responses to lower concentrations. Thus, PCE2 appeared to play a role in the ability of low concentrations of IL-I to stimulate thymidine incorporation. PGE2 augmented thymidine incorporation by increasing cAMP accumulation because in the presence of indomethacin addition of exogenous cAMP enhanced thymidine incorporation in response to low concentrations of IL-1. Elevated cAMP further stimulated PGEL synthesis. Thus, PGEz and cAMP interacted to potentiate their mutual accumulation. In summary, IL-1 stimulates PGEL synthesis. PGE2, in turn, stimulates cAMP accumulation which potentiates IL-1-stimulated PGEL synthesis and thymidine incorporation.