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Interleukin-1 induces collagenase production by recessive dystrophic epidermolysis bullosa fibroblasts

✍ Scribed by K. Nomura; T. Imaizumi; H. Mikami; K. Tamai; M. Takahashi; I. Hashimoto

Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
417 KB
Volume
282
Category
Article
ISSN
0340-3696

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✦ Synopsis

We examined the direct effect of interleukin-1 (IL-1) on the collagenase production by epidermolysis bullosa (EB) fibroblasts. Addition of IL-1 at concentrations of 2.5 x 10-4 units/ml or below in the culture media greatly enhanced collagenase production by two cell lines of recessive dystrophic EB (RDEB) fibroblasts. They produced 4.82 l+ 0.04 to 5.93 _+ 0.39 units]ml of enzyme, as compared to 0.02 + 0.07 units]ml in the absence of IL-1. In contrast, collagenase production by two cell lines of dominant dystrophic EB (DDEB) and normal fibroblasts was not, or only slightly, increased up to 0.69 _+ 0.28 units]ml. IL-1 concentrations of 2.5 x 10-s units[ml or higher failed to induce collagenase production by all fibroblasts. 3Hthymidine uptake increased by about 110-376% of control after IL-1 treatment. In addition, these data were obtained using fibroblasts of the 13-15 passages, suggesting that the property might be determined genetically. Although RDEB seems to be a wide heterogeneous group, the present data strongly suggest that the property may be specific to and characteristic of some types of RDEB cells.

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