Interleukin 1-induced cancer cell/endothelial cell adhesionin vitroand its relationship to metastasisin vivo: role of vessel wall 13-HODE synthesis and integrin expression
✍ Scribed by Maria C. Bertomeu; Sharon Gallo; Davide Lauri; Thomas A. Haas; F. William Orr; Eva Bastida; Michael R. Buchanan
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 711 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0262-0898
No coin nor oath required. For personal study only.
✦ Synopsis
Previously, we have demonstrated that stimulation of endothelial cells (ECs) with interleukin-la (IL-la) enhances the synthesis and expression of the vitronectin receptor (VnR), promotes VnR-dependent adhesion of human A549 adenocarcinoma cells to ECs, and is associated with decreased EC 13-hydroxyoctadecadienoic acid (13-HODE) synthesis in vitro. To determine whether these observations are relevant in vivo, we examined the acute retention and subsequent metastasis of intravenously-injected B16F10 melanoma cells in murine lungs, in relation to vessel wall 13-HODE. In C57BL/6 mice pretreated with IL-la, vessel wall 13-HODE was decreased and B16F10 lung entrapment and metastasis were increased. The latter two events were blocked by pretreating the animals with the GRGDS peptide. These data suggest a relationship between vessel wall 13-HODE synthesis, adhesion molecule expression, and adhesion of B16F10 cells to the endothelium.