Interleukin 1 and tumor necrosis factor-α additively increase the levels of granulocyte-macrophage and granulocyte colony-stimulating factor (CSF) mRNA in human fibroblasts

✍ Scribed by Walter Seelentag; Jean-Jacques Mermod; Pierre Vassalli

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 630 KB
• Volume: 19
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830190135

✦ Synopsis

Interleukin 1 and tumor necrosis factor-a additively increase the levels of granulocyte-macrophage and granulocyte colony-stimulating factor (CSF) mRNA in human fibroblasts* Recombinant interleukin (IL) 1 p and tumor necrosis factorkachectin (TNF-a) induce, usually within 2 h, a dose-dependent increase in the levels of granulocytemacrophage colony-stimulating factor (GM-CSF) and G-CSF mRNA in cultured human fibroblasts. Maximal induction is reached at about 4-8 h and usually last for at least 48 h. IL1p and TNF have additive effects on the levels of GM-and G-CSF mRNA, and on the secretion of G-CSF activity into the culture medium. IL la has the same additive effect that IL l p has with TNF, but no additive effect with IL lp. In contrast, the high basic level of M-CSF (CSF-1) mRNA shows little or lower variations in response to IL1, TNF-a or both IL1 and TNF-a also induce, with similar kinetics, an increase in ILlp but not mRNA level. In contrast to what is observed with macrophages and endothelial cells, E. coli lipopolysaccharide does not modify the fibroblast CSF mRNA level up to 48 h of culture.