Interlaboratory variation in oxygen tension measurement by Eppendorf “Histograph” and comparison with hypoxic marker
✍ Scribed by Nozue, Mutsumi; Lee, Intae; Yuan, Fan; Teicher, Beverly A.; Brizel, David M.; Dewhirst, Mark W.; Milross, Christopher G.; Milas, Luka; Song, Chang W.; Thomas, Carole D.; Guichard, Marcelle; Evans, Sydney M.; Koch, Cameron J.; Lord, Edith M.; Jain, Rakesh K.; Suit, Herman D.
- Book ID
- 101218870
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 407 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0022-4790
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✦ Synopsis
Background and Objectives:
The median of pO 2 values in tumor measured by Eppendorf ''Histograph'' with a needle-type electrode has been used as a prognostic indicator in cancer patients. However, it is not established that a pretreatment measured pO 2 value can be used as a universal predictor of local control probability, because the variation in pO 2 values, especially in hypoxic tissue, among institutes may not allow comparison of measured ''absolute pO 2 values.'' The purpose of this study was to examine the variation in oxygen tension measurement by Eppendorf ''Histograph'' among six laboratories using a single batch of mice and tumors and the same detailed protocol. These results were also compared to the immunohistochemical staining of 2-nitroimidazole adducts. Methods: C3H mice bearing FSaII murine fibrosarcoma subcutaneously were shipped to all laboratories, and the oxygen status in tumors and in normal subcutis was examined using Eppendorf ''Histograph'' and immunohistochemical hypoxic marker. Results: All laboratories showed that the FSaII tumor was hypoxic with at least 77% of measured points under 10 mmHg in pO 2 and with a median pO 2 value less than that of normal subcutis. These results were further confirmed immunohistochemically. These findings are interpreted as evidence that the pO 2 values measured by Eppendorf ''Histograph'' can be useful. However, the median values of tumor pO 2 varied from 1.5 mmHg