Interlaboratory concordance of DNA sequence analysis to detect reverse transcriptase mutations in HIV-1 proviral DNA
โ Scribed by Lisa M. Demeter; Richard D'Aquila; Owen Weislow; Eric Lorenzo; Alejo Erice; Joseph Fitzgibbon; Robert Shafer; Douglas Richman; Thomas M. Howard; Yuqi Zhao; Eva Fisher; Diana Huang; Douglas Mayers; Shelly Sylvester; Max Arens; Kim Sannerud; Suraiya Rasheed; Victoria Johnson; Daniel Kuritzkes; Patricia Reichelderfer; Anthony Japour
- Book ID
- 114140654
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 139 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0166-0934
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An internally controlled high-input PCR method, termed HIV-1 Mega-PCR was developed to lower the detection limit of HIV-1 DNA polymerase chain reaction (PCR) and to improve its value as a complementary diagnostic test. It is based on PCR amplification of two target sequences in the gag gene of HIV-1