Intergeneric gene transfer of six isozyme loci in rainbow trout by sperm chromosome fragmentation and gynogenesis
✍ Scribed by Disney, Jane E. ;Johnson, Kenneth R. ;Thorgaard, Gary H.
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 899 KB
- Volume
- 244
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Transgenic rainbow trout were produced by fertilizing albino rainbow trout eggs with gamma-irradiated brook trout (ST) or rainbow trout (RT) sperm and then heat shocking the eggs at 29°C for 10 minutes to induce second polar body retention. Electrophoretic analyses of the RT x ST crosses confirmed the expression of paternal (brook trout specific) alleles at Ldh-4, Mdh-3,4, Mdh-2, Aat-1,2, Gpi-3, and 6-Pgd; the transfer frequency of these loci varied from 2 to 17%. These loci range from 8 to 48 cM from the centromere; proximity of introduced genes to a centromere does not appear to be a factor in retention and expression of foreign genes. Cytogenetic analyses of these same crosses revealed variable numbers and types of chromosome fragments and rings within individuals. Survival and pigment transfer in the resultant gynogenetic offspring varied considerably due to the individual female used in a particular cross. This female effect may have obscured effects due to dose of irradiation and species of male.