Interferon-γ regulates expression of a novel keratin classe I gene

✍ Scribed by Thomas Flohr; Ute Buwitt; Bernd Bonnekoh; Thomas Decker; Erik C. Böttger

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 557 KB
• Volume: 22
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830220415

✦ Synopsis

Interferon-y regulates expression of a novel keratin classe I gene*

Interferon (1FN)-y has been implicated in the pathogenesis of several autoimmune disorders and inflammatory skin diseases. To identify novel mediators involved in the IFN-y response we have used differential hybridization of a cDNA library prepared from IFN-y-treated HeLa cells to isolate a gene that is induced following treatment with IFN-y. We report here the molecular cloning and characterization of a cDNA detecting a 1.6-kb mRNA that accumulated in response to IFN-y but not in response to IFN-a or IFN-fi.The gene is regulated by IFN-y in human cell lines of epithelial origin. The mRNA encodes a predicted protein of 432 amino acids and the primary structure of the protein demonstrates that it is a novel member of developmentally regulated keratin class I genes.

* This work was supported by SFB 244 from the Deutsche Forschungsgemeinschaft (E. C. Bottger).