Interferon-Gamma inhibits proliferation, but not commitment, of murine granulocyte-macrophage progenitors

✍ Scribed by Kenichi Koike; Feng Ma; Masaaki Shiohara; Tetsuo Kubo; Mineo Takagi; Yoshiro Amano; Tatsutoshi Nakahata; Atsushi Komiyama

• Book ID: 102882698
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 615 KB
• Volume: 153
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041530313

✦ Synopsis

Abstract

We investigated the effects of interferon γ (IFN‐γ) on the growth of murine hematopoietic progenitors. IFN‐γ inhibited granulocyte colony‐stimulating factor (G‐CSF)‐ and interleukin‐3 (IL‐3)‐dependent colony growth by granulocyte‐macrophage (GM) progenitors derived from the bone marrow cells of normal mice. However, the number of IL‐3‐dependent GM colonies formed by the bone marrow cells of 5‐fluorouracil (5‐FU)‐treated mice was not influenced by the addition of IFN‐γ. Replating experiments suggested that IFN‐γ suppressed GM colony growth directly and that it exerted an inhibitory effect on the proliferation, but not on the commitment, of GM progenitors. In contrast, IFN‐γ failed to suppress colony growth by mast cell progenitors. Erythroid and megakaryocytic progenitors exhibited different responses to IFN‐γ depending on mouse strains. These results suggest that potent negative regulators are not always inhibitors of hematopoietic progenitors. © 1992 Wiley‐Liss, Inc.