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Interferences in deoxyribose determination by the cysteine-HCl sulphuric acid method

โœ Scribed by Luigi Messineo

Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
521 KB
Volume
3
Category
Article
ISSN
0020-711X

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Elimination of 2-deoxyribose interferenc
Elimination of 2-deoxyribose interference in the thiobarbituric acid determination of N-acetylneuraminic acid in tumor cells by pH-dependent extraction with cyclohexanone
โœ John Roboz; Maitree Suttajit; J. George Bekesi ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 739 KB

The widely used thiobarbituric acid technique for the quantitation of N-acetylneuraminic acid (NANA) was improved to eliminate the interference of the ubiquitous 2-deoxyribose. The 2-deoxyribose chromogen was completely removed by cyclohexanone extraction at pH 5.6-6.0. After readjusting the pH to 1