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Interference of Good's buffers and other biological buffers with protein determination

✍ Scribed by P.L. Lleu; G. Rebel

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
291 KB
Volume
192
Category
Article
ISSN
0003-2697

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✦ Synopsis

Interference of low concentrations of Hepes and other buffers commonly used in protein determination was studied. The data show that some of these buffers interfere to differing degrees with protein determination according to the Lowry method. A study of the structure-interference relationship suggests that the group ethanolamine is involved in this interference. No interference was observed when protein was measured using bicinchonic acid at the same concentration as the Lowry reagent.

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