Interference of dexamethasone with leukocyte blood volume and albumin movements in lungs from endotoxemic guinea-pigs
✍ Scribed by C.M.M.B. de Castro; M.F. Bureau; B.B. Vargaftig; M. Bachelet
- Publisher
- Elsevier
- Year
- 1995
- Tongue
- English
- Weight
- 777 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0952-0600
No coin nor oath required. For personal study only.
✦ Synopsis
Using radioactive tracers, we measured blood volume, albumin exchanges and blood leukocyte sequestration within lungs, following an intravenous injection of lipopolysaccharide (0.1-1 mg/kg). Neutrophil infiltration into the airways was followed in parallel experiments. Dexamethasone pretreatment (20 mg/kg, subcutaneous) failed to prevent early pulmonary changes induced by lipopolysaccharide as decreased blood volume, leukocyte sequestration, leukopenia or the increased trans-endothelial albumin exchanges. However, dexamethasone provided a significant protection against the later albumin leakage through the endothelial/epithelial barrier and the neutrophil accumulation in the airways observed in lipopolysaccharide-treated guinea-pigs. Our results indicate that the protective effect of dexamethasone in lipopolysaccharide-induced lung injury might derive from an initial reduction of leukocyte adhesion and a later decrease in alveolo-capillary permeability.