Interest and limitations of Spliced Leader Intergenic Region sequences for analyzing Trypanosoma cruzi I phylogenetic diversity in the Argentinean Chaco
✍ Scribed by Nicolás Tomasini; Juan J. Lauthier; María M. Monje Rumi; Paula G. Ragone; Anahí A. Alberti D’Amato; Cecilia Pérez Brandan; Carolina I. Cura; Alejandro G. Schijman; Christian Barnabé; Michel Tibayrenc; Miguel A. Basombrío; Alejandra Falla; Claudia Herrera; Felipe Guhl; Patricio Diosque
- Book ID
- 104050747
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 372 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1567-1348
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✦ Synopsis
Internal and geographical clustering within Trypanosoma cruzi I (TcI) has been recently revealed by using Multilocus Microsatellite Typing and sequencing of the Spliced-Leader Intergenic Region (SL-IR). In the present work, 14 isolates and 11 laboratory-cloned stocks obtained from a geographically restricted area in Chaco Province, Argentina, were analyzed by PCR and sequencing of SL-IR. We were able to differentiate 8 different genotypes that clustered into 4 groups. One of these groups was classified within the formerly described haplotype A and another one within the recently described SL-IR group E. Both were phylogenetically well-supported. In contrast, none of the stocks from the Chaco province were grouped within the cluster previously named haplotype D despite the fact that they shared a similar microsatellite motif in the SL-IR. No evidence of recombination or gene conversion within these stocks was found. On the other hand, multiple ambiguous alignments in the microsatellite region of SL-IR, affecting the tree topology and relationships among groups were detected. Finally, since there are multiple copies of the SL-IR, and they are arranged in tandem, we discuss how molecular processes affecting this kind of sequences could mislead phylogenetic inference.