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Interdomain Dynamics of Phosphoglycerate Kinase Studied by Single-Molecule FRET and a Mesoscale Hydrodynamics Simulation

✍ Scribed by Gabba, Matteo; Poblete, Simon; Kempe, Daryan; Schöne, Antonie; Züchner, Tina; Gompper, Gerhard; Fitter, Jörg

Book ID: 122298350
Publisher: Biophysical Society
Year: 2014
Tongue: English
Weight: 46 KB
Volume: 106
Category: Article
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.1488

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## Abstract Single‐molecule Förster resonance energy transfer (FRET) measurements with phosphoglycerate kinase from yeast were performed at different concentrations of guanidine hydrochloride. From these steady‐state measurements we obtained FRET efficiency histograms characterizing structural prop