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Interconversion of gibberellin A4to gibberellins A1and A34by dwarf rice, cultivar Tan-ginbozu

โœ Scribed by Richard C. Durley; Richard P. Pharis


Book ID
104750547
Publisher
Springer-Verlag
Year
1973
Tongue
English
Weight
291 KB
Volume
109
Category
Article
ISSN
0032-0935

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โœฆ Synopsis


Interconversion of GA4 to GA1 and GA34 occurred within 24 h of application of 1,2-[(3)H]-GA4 to seedlings of dwarf rice, cv. Tan-ginbozu. Identification was made by direct comparison of the trimethylsilyl ether derivatives of the methyl esters of Silica-gel partition-column fractions on gas-liquid radiochromatography with derivatized GA1 and GA34 standards on three columns: 2% QF-1, 2% SE-30, and 1% XE-60. GA2, an artifact of the purification and chromatography system, may also be formed by the plant. The conversions from GA4 to GA1 and GA34 are single hydroxylations. At least two unidentified radioactive products were also formed by the plant. Interconversions were in the order of 0.3 to 0.8% of applied [(3)H]-GA4.


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When barley aleurone layers were incubated with (3)H-Gibberellin A1 ((3)H-GA1), the hormone was converted to (3)H-GA-X (not identified), (3)H-GA8 and two other compounds tentatively identified as (3)H-GA1-glucoside, and (3)H-GA8-glucoside. Uptake and metabolism of the (3)H-GA1 were markedly enhanced