Interconversion of gibberellin A4to gibberellins A1and A34by dwarf rice, cultivar Tan-ginbozu
โ Scribed by Richard C. Durley; Richard P. Pharis
- Book ID
- 104750547
- Publisher
- Springer-Verlag
- Year
- 1973
- Tongue
- English
- Weight
- 291 KB
- Volume
- 109
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
โฆ Synopsis
Interconversion of GA4 to GA1 and GA34 occurred within 24 h of application of 1,2-[(3)H]-GA4 to seedlings of dwarf rice, cv. Tan-ginbozu. Identification was made by direct comparison of the trimethylsilyl ether derivatives of the methyl esters of Silica-gel partition-column fractions on gas-liquid radiochromatography with derivatized GA1 and GA34 standards on three columns: 2% QF-1, 2% SE-30, and 1% XE-60. GA2, an artifact of the purification and chromatography system, may also be formed by the plant. The conversions from GA4 to GA1 and GA34 are single hydroxylations. At least two unidentified radioactive products were also formed by the plant. Interconversions were in the order of 0.3 to 0.8% of applied [(3)H]-GA4.
๐ SIMILAR VOLUMES
Cell-free systems were prepared from germinating seed and seedlings of Phaseolus coccineus. Gibberellin A4 (GA4)-metabolising activity was detected in vitro using preparations from roots, shoots and cotyledons of germinating seed, but only up to 24 h after imbibition. Cell-free preparations from cot
When barley aleurone layers were incubated with (3)H-Gibberellin A1 ((3)H-GA1), the hormone was converted to (3)H-GA-X (not identified), (3)H-GA8 and two other compounds tentatively identified as (3)H-GA1-glucoside, and (3)H-GA8-glucoside. Uptake and metabolism of the (3)H-GA1 were markedly enhanced