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Intercellular NOR-AG variability in man

✍ Scribed by O. A. Sozansky; A. F. Zakharov; V. A. Benjush

Book ID
104708109
Publisher
Springer
Year
1984
Tongue
English
Weight
398 KB
Volume
68
Category
Article
ISSN
0340-6717

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✦ Synopsis

A new modification of the Ag I technique has been developed using human cultured blood lymphocytes, which involves ultra-violet irradiation of chromosome preparations during incubation in AgNO3. This technique enables detection in a short incubation time all NORs capable of being stained with silver. A peculiar morphological change in Ag-stainable NORs during the incubation is described, which can be used as a criterion of the completion of Ag staining. With the refined Ag-staining procedure, acrocentric marker chromosomes were studied which showed one or two satellite stalks within the same individual. Ag staining was highly coincident with this variability.

πŸ“œ SIMILAR VOLUMES

Intercellular NOR-Ag-variability in man.
Intercellular NOR-Ag-variability in man. II. Search for determining factors, clonal analysis
✍ O. A. Sozansky; A. F. Zakharov; S. M. Terekhov πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 652 KB

Intercellular, nonartifactual variability of nucleolar organizer region (NOR)-Ag-staining was studied in cultured human peripheral blood lymphocytes, skin and embryonic fibroblasts. No differences in number and character of variable NORs and intensity of their staining were observed between lymphocy