Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation
✍ Scribed by Luca Butini; Antonin R. De Fougerolles; Mauro Vaccarezza; Cecilia Graziosi; David I. Cohen; Maria Montroni; Timothy A. Springer; Giuseppe Pantaleo; Anthony S. Fauci
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 677 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Intercellular adhesion molecules (ICAM) -1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation*
It has been previously demonstrated that lymphocyte function-associated molecule 1 (LFA-1) plays a major role in human immunodeficiency virus (HIV)-mediated syncytia formation. In the present study we investigated the involvement of intercellular adhesion molecule-1 (ICAM-l), ICAM-2 and ICAM-3 in the process. The ability of monoclonal antibodies (mAb) directed against ICAM-1, ICAM-2 and ICAM-3 to block syncytia was analyzed either in phytohemagglutinin (PHA)-activated lymphocytes infected in v i m with primary or laboratory strains of HIVor by coculturing aTcell line stably expressing HIV envelope with PHA-activated lymphocytes. Complete inhibition of syncytia formation was observed only by the simultaneous addition to the cell cultures of all (i.e. anti-ICAM-1, anti-ICAM-2 and anti-ICAM-3) mAb. These results indicate that the interaction between LFA-1 and ICAM is a critical step in HIV-mediated syncytia formation, and that ICAM-1, ICAM-2 and ICAM-3 are the receptor molecules for the LFA-l-dependent syncytia formation.