Interactive computer-aided assignment of multiple probes to cytogenetic bands by simultaneous dual color fluorescence in situ hybridization and DAPI banding
✍ Scribed by Stefan Burde; Greg Joss; Julia A. Gonzales; Christopher H. Coulon; Min S. Park; Larry L. Deaven; Babetta L. Marrone
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 822 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
A macro function was developed to run in conjunction with the popular image analysis package NIH Image, to allow simultaneous determination of mapping positions of one or two separate probes with respect to cytogenetic bands by dual color fluorescence in situ hybridization (FISH) and DAPI banding, and by determination of their fractional distance from pter (FLpter). In order to allow maximal flexibility, a user-defhed line along the chromosome is used for measurements. Algorithms were developed to detect the ends of the chromosome and the cytogenetic bands. Results of the anal-