Interactions of MAP/microtubule affinity regulating kinases with the adaptor complex AP-2 of clathrin-coated vesicles
✍ Scribed by Schmitt-Ulms, Gerold ;Matenia, Dorthe ;Drewes, Gerard ;Mandelkow, Eva-Maria
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 470 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0886-1544
- DOI
- 10.1002/cm.20394
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✦ Synopsis
Abstract
MARK [microtubule‐associated proteins (MAPs)/microtubule affinity regulating kinase]/Par‐1 (partition defective) phosphorylate MAPs tau, MAP2 and MAP4 at KXGS motifs and thereby regulate microtubule dynamics and transport processes in cells [Drewes et al., Cell1997;89:297–308]. We report here that MARK copurifies with clathrin‐coated vesicles (CCVs) via interaction with the adaptor complex AP‐2. The adaptin binding site on MARK includes the regulatory loop of its catalytic domain. Immunofluorescence demonstrates the colocalization of MARK with AP‐2 and clathrin, as well as other MARK‐interacting proteins such as PAK5. The results are consistent with an observed influence of MARK on the trafficking of CCVs. Cell Motil. Cytoskeleton 2009. © 2009 Wiley‐Liss, Inc.