Interactions of drugs and an oligonucleotide with charged membranes analyzed by immobilized liposome chromatography
✍ Scribed by Anna Lundquist; Caroline Engvall; Elisabet Boija; Sanela Kurtovic; Jyoti Chattopadhyaya; Christine Lagerquist Hägglund; Per Lundahl§
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 131 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0269-3879
- DOI
- 10.1002/bmc.532
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✦ Synopsis
We studied the effect of charged lipids or detergent on the retention of drugs and an oligonucleotide by immobilized liposome chromatography to characterize solute-membrane interactions. This is a novel approach in analysis of oligonucleotide-liposome interactions. The charged lipids (phosphatidylserine or distearoyltrimethylammoniumpropane) or detergent (sodium dodecylsulfate) interacted electrostatically in a concentration-dependent matter with the solutes. The oligonucleotide ions presumably bound to the liposomes by multipoint interactions, which was saturable. Sodium dodecylsulfate seemed to affect the drug-membrane interactions more strongly than phosphatidylserine did, probably due to different positioning in the bilayer.