Interactions between chromosomal omnipotent suppressors and extrachromosomal effectors inSaccharomyces cerevisiae
✍ Scribed by Bun-ichiro Ono; Yury O. Chernoff; Yumiko Ishino-Arao; Noriko Yamagishi; Sumio Shinoda; Sergey G. Inge-Vechtomov
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 550 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0172-8083
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✦ Synopsis
Chromosomal omnipotent suppressor mutations recovered in ~ + strains of Saccharomyces cerevisiae were brought into ~-cytoplasm. SUP46, SUP138 and SUP139 acted as dominant omnipotent suppressors in the (/-cytoplasm though their suppressor activity was substantially reduced. SUP46 and SUP138 conferred recessive thermosensitivity and antibiotic sensitivity in ~cytoplasm as in ~ + cytoplasm. On the other hand, suplll through supll5, which acted as recessive omnipotent suppressors in the ~ + cytoplasm, manifested no, or very low, suppressor activity in the ~-cytoplasm. They, however, still enhanced the efficiency of the SUP29 tRNA suppressor in ~9-cytoplasm. A multicopy plasmid carrying the wild-type SUP35 gene enhanced the efficiency of suplll in ~-cytoplasm.