Interaction of the anti-tumour drug cis-Pt(en)Cl2 with cytidilyl-3′ → 5′-guanosine

✍ Scribed by J. Jordanov; R.J.P. Williams

• Publisher: Elsevier Science
• Year: 1978
• Weight: 279 KB
• Volume: 8
• Category: Article
• ISSN: 0006-3061
• DOI: 10.1016/s0006-3061(00)80006-x

✦ Synopsis

Chemical shifts occurring in lH nuclear magnetic resonance and Sephadex G 2.5 chromatography are used to follow the reaction of the dinucleotide C3'pSG (cytidilyl--3'phosphateS'-o,uanosine) in aqueous solution and to separate its products. Binding of the platinum occurs in first place at the cytosine, then at the guanine base. TWO major complexes are produced, Pt:CpG and (pt:CpG)2, which account for respectively for an internal and an external cross-linking effect_