Interaction of poly(A) with different adsorbents for affinity chromatography of nucleic acids
โ Scribed by J.-L. Ochoa; J. Kempf; J.M. Egly
- Book ID
- 119069614
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 619 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0141-8130
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๐ SIMILAR VOLUMES
Republic of Germany ## Synopsis The affinity of amino acid residues to nucleic acids is probed by measurements of melting temperatures t , for the helix-coil transition a t various concentrations of amino acid amides. The increase oft, on addition of ligand is described by the equation t , = t k
The optical density-temperature profile of double-stranded poly(A + U), triplestranded poly(A + 2U), and double-stranded RNA from rice dwarf virus in solutions with and without poly-clysine has been examined. When poly-clysine is added, more than one melting temperature T , is observed for poly(A +