Interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine

✍ Scribed by Stanislava Rybáková, Michal Rájecký, Jana Urbanová, Kristýna Pěnčíková…

• Book ID: 120948630
• Publisher: Versita
• Year: 2013
• Tongue: English
• Weight: 197 KB
• Volume: 67
• Category: Article
• ISSN: 0366-6352
• DOI: 10.2478/s11696-013-0340-x

✦ Synopsis

Benzo[c]phenanthridine alkaloid sanguilutine was extracted and purified from the dried roots of Sanguinaria canadensis. The interaction of the positively charged iminium form of alkaloid with double-stranded DNA oligonucleotides was studied using luminescence spectroscopy. The results showed that the interaction with various double-stranded oligonucleotides was not specific to A-T or G-C base pairs; also, no preference was found for either homogeneous or heterogeneous base composition of strands. The association constants were calculated to be in the range of (1.31-14.36) × 10 5 M -1 . The luminescence intensity response at 610 nm to low concentrations of double-stranded DNA was found to be linear and can potentially be used for the fluorometric quantification of DNA. The limit of detection was estimated to be 120 ng mL -1 of DNA (calculated by 3σ method).