Interaction of mithramycin with isolated GC and CG sites

✍ Scribed by Mark L. Carpenter; Sarah A. Cassidy; Keith R. Fox

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 793 KB
• Volume: 7
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/jmr.300070306

✦ Synopsis

Abstract

We have studied the interaction of the GC‐specific, minor groove‐binding ligand, mithramycin, with cloned DNA inserts containing isolated GC and CG sites flanked by regions of (AT)~n~ and A~n~ · T~n~ using DNase I and hydroxyl radical footprinting. We find that mithramycin binds to GC better than CG and that AGCT is a better site than TGCA. Sites flanked by (AT)~n~ appear to be bound better than those surrounded by A~n~ · T~n~. Although no footprints are produced at T~9~GCA~9~ and T~15~CGA~15~, DNase I cleavage is enhanced with the GC sites suggesting that there is some interaction with the ligand. Mithramycin also alters the DNase I cleavage of (GA)~n~ · (CT)~n~.