Interaction of long-chain choline derivatives with firefly luciferase and their use as reagents for the extraction of intracellular ATP from microorganisms
✍ Scribed by Alexey Dukhovich; M. Jesus Rodriguez; L. Gerardo Gaona; Andrei Levashov; Natalia Ugarova
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 505 KB
- Volume
- 303
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Various long-chain choline derivatives, differing in the length of the hydrocarbon backbone, have been synthesized. The influence of these compounds on the activity of firefly luciferase has been studied. Whereas C.&u derivatives do not influence enzyme activity, C,,-C,, derivatives inactivate luciferase rapidly and irreversibly. It is suggested that interaction between the enzyme and these compounds has a hydrophobic nature. Phospholipids stabilize luciferase against the inactivation by these derivatives. It was shown that long-chain choline derivatives with a backbone of 10 carbon atoms and more can be used for ATP extraction and further bioluminescent microassay in the presence of phosphohpid additives as the agents which stabilize luciferase.