Interaction of glycyrol with calcineurin A studied by spectroscopic methods and docking

✍ Scribed by Liqin Peng; Yao Qi; Hezhen Wu; Qun Wei

• Book ID: 102870270
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 860 KB
• Volume: 63
• Category: Article
• ISSN: 1521-6543
• DOI: 10.1002/iub.408

✦ Synopsis

Abstract

We have shown previously that glycyrol has an inhibitory effect on the immune response in mice by reducing calcineurin activity (Li et al., 2010, Pharm Biol 48:1177–1184). Here, we investigated the interaction of glycyrol with calcineurin A (CNA, catalytic A subunit of calcineurin) by spectroscopic methods and docking. We showed that glycyrol binds to CNA via hydrophobic interactions in a ratio of 1:1, and the main binding site is in the catalytic domain of CNA close to the calcineurin B subunit‐binding domain. Binding of glycyrol changes the secondary structure of CNA, and this effect may possibly inhibit CN activity. © 2011 IUBMB IUBMB Life, 63(1): 14–20, 2011