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Interaction of exogenous DNA with the nuclear matrix of live spermatozoa

✍ Scribed by Siobhan McCarthy; W. Steven Ward

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
64 KB
Volume
56
Category
Article
ISSN
1040-452X

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✦ Synopsis

Sperm chromatin is a highly organized array of protamines and DNA, with the protamines serving to tightly condense the DNA into a compact, defined structure. We have previously demonstrated that the sperm nucleus is an ordered library of DNA organized into functional zones, such as the nuclear matrix and nuclear annulus. Other laboratories have suggested that mouse spermatozoa can interact with exogenous pSV2CAT plasmid DNA. In this work, we explored this interaction and examined the subcellular localization of the exogenous DNA. We found a repeatable association of exogenous DNA with a specific region of the sperm nuclear matrix. This region of the nucleus correlates with the equatorial segment of the sperm head. This interaction requires only a defined fertilization media, transfection quality DNA, and incubation with spermatozoa.

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