Interaction of 1-anilinonaphthalene 8-sulfonic acid with interfaces containing cerebrosides, sulfatides and gangliosides

✍ Scribed by Guillermo G. Montich; Juan J. Cosa; Bruno Maggio

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 568 KB
• Volume: 49
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(88)90072-2

✦ Synopsis

The fluorescence lifetime, quantum yield and emission spectra of 1-anilinonaphthalene 8-sulfonic acid (ANS) associated with interfaces of pure dipalmitoylphosphafidylcholine or its mixtures with phosphatidylserine, galactosylceramide, sulfatide or gangliosides GM1 and Gin. were studied at low and high ionic strength. Modification of the molecular organization of the lipid interfaces in the presence of the probe was also studied with mixed lipid monolayers. ANS has little affect on the intermolecular packing of the lipids but influences their surface potential, consistent with a location of ANS in the polar head group region of the interface. ANS senses a more polar microenvironment when associated with interfaces containing anionic glycosphingolipids at low ionic strength but, except for interfaces containing phosphatidylserine, it detects approximately the same polarity for neutral or anionic interfaces in 0.25 M NaCI.