✍ Scribed by Sabine Päuser; Adolf Zschunke; Konrad Keller
No coin nor oath required. For personal study only.
Xenopus lamis were used as a well-established model to investigate spatially resolved changes in relaxation time (Tl) within a cell during exposure to copper complexes by means of 'H-NMR-microscopy. T1 relaxation of intracellular water was shortened in dependence on the complex concentration, the water content, and the water mobility in various cell compartments. A relatively constant T1 decrease was observed in the cytoplasm of the vegetal pole, irrespective of the type and concentration of the permeable complexes used. Since the lowest content of free mobile water molecules was detected at the vegetal pole, we concluded that this quantity was as important for the relaxation time decrease as that of the chelated paramagnetic ions. Experiments using l*C-labeled inulin demonstrated that the paramagnetic metal complexes entered the oocytes without gross injury to ~ their plasma membranes. Index terms: NMR microscopy -Xenopus laeuls oocyte * Contrast agent in teraction mth intracellular water
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## Abstract The synthesis, crystal structure and variable‐temperature magnetic measurements of compounds [M~2~(μ‐Hade)~2~(μ‐mal)~2~(H~2~O)~2~]·2H~2~O [M = Ni (1), Co (2)], [Co~2~(μ‐Hade)~2~(μ‐mal)~2~(H~2~O)~2~] (3), {(H~2~ade)~2~[Cu(μ‐mal)~2~]·2H~2~O}__~n~__ (4) and (H~2~ade)~2~[Cu(mmal)~2~(H~2~O)]