Interaction between insulin receptors and major histocompatibility complex antigens in mouse liver membranes

✍ Scribed by N. Brossette; E. Van Obberghen; M. Fehlmann

• Book ID: 104698524
• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 426 KB
• Volume: 27
• Category: Article
• ISSN: 0012-186X
• DOI: 10.1007/bf00275651

✦ Synopsis

Insulin receptors from H-2k mouse liver membranes were specifically labelled using a photoreactive insulin analogue coupled to the alpha-subunit (Mr 130,000) of the receptor by ultraviolet-irradiation. Up to 25% of the labelled insulin receptors recovered after membrane solubilization and precipitable by anti-insulin receptor antibodies could be immunoprecipitated by various monoclonal antibodies reacting with H-2Kk antigens. None of these monoclonal antibodies inhibited insulin binding to its receptor. These results show that insulin receptors and H-2Kk antigens interact in liver plasma membranes to form non-covalent complexes. This association between insulin receptors and major histocompatibility complex antigens could be involved in the pathogenesis of certain forms of insulin resistance.