Interaction between aflatoxin B1and oxytetracycline in isolated rat hepatocytes

✍ Scribed by Marie N. Blaude; Fabienne M. Goethals; Michel A. Ansay; Marcel B. Roberfroid

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 594 KB
• Volume: 6
• Category: Article
• ISSN: 0742-2091
• DOI: 10.1007/bf00120801

✦ Synopsis

Isolated rat hepatocytes were used as an in vitro model to investigate a possible interaction between oxytetracycline (OXT) and aflatoxin B1 (AFB1). L D H leakage, R N A and protein synthesis and glycogen accumulation were measured in the presence of both drugs, either separately or in combination. The evolution o f L D H leakage during the incubation was identical in untreated and treated cells. AFB1 inhibited R N A and protein synthesis at a con-

centration of lO-ZM and 10-6M, respectively, and higher, whereas O X T did not influence R N A synthesis but inhibited protein synthesis at the highest tested concentration, lO-3M. As far as glycogen is concerned, rats were injected with glucagon before sacrifice in order to obtain a constant synthesis rate in isolated hepatocytes. AFB1 inhibited the accumulation of glycogen from lO-6M upward. This effect was never observed before 90 min o f incubation. O X T had no effect on glycogen synthesis. In the presence of both drugs, no interaction was demonstrated as far as R N A and protein synthesis were concerned, but O X T opposed the inhibition induced by AFB1 on glycogen accumulation. If the "'in vivo "protection, provided