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Intensity fluctuation spectroscopy of deoxyribonucleic acid (DNA). I. Temperature profile of bacteriophage N1-DNA

✍ Scribed by R. L. Schmidt; J. A. Boyle; John A. Mayo

Publisher: Wiley (John Wiley & Sons)
Year: 1977
Tongue: English
Weight: 606 KB
Volume: 16
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1977.360160208

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✦ Synopsis

Abstract

Intensity‐fluctuation laser‐light‐scattering spectroscopy has been used to characterize the temperature dependence of the translational diffusion coefficient through the helix–coil region for the DNA isolated from bacteriophage N1. The diffusion coefficient does not change smoothly, but exhibits fine structure detail similar to that seen in the uv absorption hyperchromicity. These changes are related to the heterogeneous distribution of bases along the N1‐DNA chain.

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Intensity fluctuation spectroscopy of deoxyribonucleic acid (DNA). II. Temperature profile of bacteriophage ϕ29-DNA

✍ R. L. Schmidt; M. A. Whitehorn; John A. Mayo 📂 Article 📅 1977 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 825 KB

## Abstract Intensity‐fluctuation laser‐scattering spectroscopy measurements have been reported for the DNA isolated from bacteriophage ϕ29. The cumulant method of data analysis gives well‐defined weighted average decay frequencies. Available theoretical models are shown to yield good values for th