Integrative refolding and purification of histidine-tagged protein by like-charge facilitated refolding and metal-chelate affinity adsorption
โ Scribed by Liu, Hu; Du, Wen-Jie; Dong, Xiao-Yan; Sun, Yan
- Book ID
- 121749393
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 704 KB
- Volume
- 1344
- Category
- Article
- ISSN
- 1873-3778
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## Abstract Metal chelate affinity precipitation (MCAP) has been successfully developed as a simple purification process for proteins that have affinity for metal ions. The present lack of widespread applications for this technique as compared to immobilized metal affinity chromatography (IMAC) may
The facilitated downstream processing of an intracellular, polyhistidine-tagged protein, glutathione Stransferase [GST-(His) 6 ], direct from unclarified E. coli homogenates using expanded beds of STREAMLINE chelating, has been investigated. A series of pilot experiments were used to develop prepara